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做网站公司宁波上市,手机网站模版下载,柳州住建局官网,自己做网站的费用4-Hydroxytamoxifen#xff08;4-OHT#xff0c;AbMole#xff0c;M10252#xff09;是一种选择性雌激素受体调节剂#xff08;SERM#xff09;#xff0c;在研究中主要作为雌激素受体拮抗剂发挥作用#xff0c;其作用机制涉及抑制雌激素受体活性并影响转录因子的招募[…4-Hydroxytamoxifen4-OHTAbMoleM10252是一种选择性雌激素受体调节剂SERM在研究中主要作为雌激素受体拮抗剂发挥作用其作用机制涉及抑制雌激素受体活性并影响转录因子的招募[1, 2]。4-Hydroxytamoxifen在细胞实验中常用于人乳腺癌细胞系如MCF-7、MDA-MB 231、T-47D的研究例如通过浓度梯度诱导细胞以100-1000 nM建立耐药细胞模型如MCF-7-TamR[3]。4-Hydroxytamoxifen在MCF-7细胞中可显著抑制细胞增殖并调控凋亡相关基因的表达[3]。4-Hydroxytamoxifen CAS No.68047-06-3在分子与细胞生物学中还是一种可以调控外源基因表达的常用化合物例如4-Hydroxytamoxifen通过激活CreER-T2酶介导loxP位点特异性重组实现外源基因在转基因小鼠模型中的条件性敲除或表达。在动物实验中4-Hydroxytamoxifen通过腹腔注射用于双转基因Cspg4-cre/Esr1/ROSA26Sortm14(CAG-tdTomato)小鼠模型诱导重组并触发红色荧光蛋白表达[4]。4-Hydroxytamoxifen还可用于动物肿瘤模型和某些病毒模型常见于小鼠实验。例如4-Hydroxytamoxifen4-OHTAbMoleM10252在异种移植或同种移植肿瘤模型中被用于评估抗肿瘤效果。在VSV水疱性口炎病毒模型中4-Hydroxytamoxifen作为小分子诱导剂可激活Intein剪接以调节病毒复制[5]。参考文献及鸣谢[1] Shtaiwi, A.; Adnan, R.; Khairuddean, M.; et al. Computational investigations of the binding mechanism of novel benzophenone imine inhibitors for the treatment of breast cancer.RSC advances2019,9(61), 35401-35416.[2] Somsen, B. A.; Sijbesma, E.; Leysen, S.; et al. Molecular basis and dual ligand regulation of tetrameric estrogen receptor alpha/14-3-3zeta protein complex.The Journal of biological chemistry2023,299(7), 104855.[3] Cheng, S.; Castillo, V.; Welty, M.; et al. BreastDefend enhances effect of tamoxifen in estrogen receptor-positive human breast cancer in vitro and in vivo.BMC complementary and alternative medicine2017,17(1), 115.[4] Valny, M.; Honsa, P.; Kirdajova, D.; et al. Tamoxifen in the Mouse Brain: Implications for Fate-Mapping Studies Using the Tamoxifen-Inducible Cre-loxP System.Frontiers in cellular neuroscience2016,10, 243.[5] Zhao, Z.; Wang, B.; Wu, S.; et al. Regulated control of virus replication by 4-hydroxytamoxifen-induced splicing.Frontiers in microbiology2023,14, 1112580.